The Protective Effect of BM Transplantation on Liver Tissue by a Chemical Carcinogen or γ-Radiation in Rats

Abstract

Aim of work: this work aimed to study the biochemical and histopathological changes in the liver of male albino rats post exposure to 6Gy of gamma radiation and the possible protective effect of bone marrow(BM) transplantation on the liver tissues by a chemical carcinogen ferric nitrilotriacetate (Fe-NTA) or γ- radiation in rats. Materials and methods:in this study, thirty six healthy and active male albino rats about 120 grams in body weight were used. The animals were housed in plastic cages under normal temperature, pressure, humidity and good ventilation conditions during the whole period of the experiment . The animals were fed on a standard pellet diet and water.Animals were categorized into six groups and served as the following groups: control, gamma irradiated(R), Fe-NTA, BM+R, BM + Fe-NTA and BM.+Fe-NTA+R. Results: the present results suggested that exposure to γ-radiation or Fe-NTA induced a significantly disturbance in the liver functions and structure. They increased significantly the oxidative stress and decreased significantly the antioxidants tissues and they also increased necrotic and apoptotic cells in rat’s liver tissue. Bone marrow transplantation either after whole body gamma-irradiation or Fe-NTA treatment restored the liver functions and structure.BT also ameliorated the oxidative stress and antioxidative markers. The histopathological observations recorded some amelioration in the apoptotic and necrotic evaluation in liver tissue.