The protective effect of 2, 2-dimethylthiazolidine on human peripheral blood lymphocytes in radiation-induced genetic damage

Abstract

Objective To investigate the protective effect of 2,2-dimethylthiazolidine (KFL) on human peripheral blood lymphocytes in radiation-induced genetic damage.Methods Peripheral blood samples were collected from healthy volunteers and divided into normal control group,irradiation group and KFL protective groups with four different concentrations (0.2,0.5,1,2 mmol/L).KFL was administrated 30 min before 2 Gy irradiation.Chromosome aberration analysis,micronucleus test and single cell gel electrophoresis were used to observe the protective effects of KFL on the radiation-induced genetic damage in human peripheral blood lymphocytes.Results Compared with the irradiation group,the four KFL protection groups showed significant reduction in the chromosomal aberration frequencies (t =5.34-25.48,P ＜0.05),the micronucleus cell frequencies (t =5.18-29.44,P ＜ 0.05),the micronucleus frequencies (t =4.67-12.04,P ＜ 0.05),the tailleghth (t =16.18-19.64,P ＜ 0.05),the tailmoment (t =11.79-13.01,P ＜ 0.05),the olivetailmoment (t =12.44-14.88,P ＜ 0.05) and the tail DNA％ of comet cells (t =12.05-17.30,P ＜ 0.05).The olivetailmoment of the two high concentrations in protective groups(1,2 mmol/L) were significantly decreased compared with the other two KFL groups (0.2,0.5 mmol/L),respectively (t =5.67-16.56,P ＜ 0.05).Compared with other KFL groups,the micronucleus frequencies and micronucleus cell frequencies of the 2 mmol/L KFL group was significantly decreased (t =4.23-5.57,P ＜ 0.05).Conclusions KFL may have protective effect on human peripheral blood lymphocytes in the genetic damage induced by radiation. Key words: 2,2-dimethylthiazolidine; Chromosome aberration ; Micronucleus ; Single cell gel electrophoresis; Radioprotection