The protection of neuregulin-1 following acute optic nerve injury in rat

Abstract

Objective This research set up the model of acute optic nerve ring clamp injury,And inject variation dosage of NRG-1 in vitreous chamber,to study the possible mechanism and the interaction among them.Methods Disregarding gender,100 matured rats were medially divided into 5 groups randomly.common group (n=20),acute optic nerve wound group;all left eye of experimental animal for operation,right eye do nothing,to build model as part 1,after injury neuregulin-1 0.5ug,1ug,3ug(each group n=20) were injcoted into vitreous chamber one time respectively,isometrical NS were injected in the control group (n=20).After ring clamp 1d,2d,7d,14d,and 28d every group professed FVEP and fundus photography to qualitation optic nerve damage and reparation.1d,2d,7d,14d,and 28d after operation,experimental animal was put to death,eyeballs involving ON were obtained tO evaluate the damage using light microscope after HE staining and TUNEL to analyze the changes of RGCs and its apoptosis compared with the control group in vitro after execution.Results Morphous,electrophysiology,pathology observation after building model.The expression of NRG-1:Compared to sham operated group,2h,1d,2d,7d,14dafter acute ON injury,The expression of NRG-1 at each time point was obviously increasing(P 0.05).Conclusions NRG-1 can effectively reduce the apoptosis of nerve cell and retina tissue,this result suggests that NRG-1 may play essential role in repairing and protecting acute optic nerve injury.It is critical to reduce the damage of optic nerve injury and protect RGCs from apoptosis. Based on our results,it is better to apply NRG-1 as early as possible after acute optic nerve injury. Key words: NRG-1; Optic nerve injury; Nerve protect; Apoptosis