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Abstract
This experiment was conducted to characterize potential Lactobacillus spp. isolated from mother's milk and infant feces to obtain new and specific probiotic strains. In this study, seven ascendant strains were identified as Lactobacillus spp. based on their morphological characteristics and biochemical properties. Among them, only one (C-1) isolate was identified as Lactobacillus oris through BioLogTM identification. The study further investigated the isolate through probiotic potentiality tests such as pH and bile tolerance, NaCl tolerance test, gastric juice tolerance, antioxidant activity, resistance to hydrogen, reduction of sodium nitrate, antimicrobial activity, and antibiotic susceptibility test. The result showed that the strain is a potential probiotic based on probiotic capability. The identified strain was most acid-tolerant and retained around 80% viability for up to 4 h at pH 1.0 and 2.0. The isolate showed tolerance against up to 1.50% bile concentration and gastric juice and was able to grow 1–6% NaCl concentrations. Lactobacillus oris showed resistance to most antibiotics as well as antagonistic activity against the tested pathogen, good antioxidant properties, reduction of sodium nitrate and H2O2. The isolate exhibited good intestinal epithelial adhesion properties, and SDS page was performed for secreted protein analysis. Moreover, the strain showed promising cholesterol-lowering properties based on the cholesterol level. This present result indicates that L. oris has superior probiotic properties and can be regarded as a potential probiotic candidate.
Highlights
Probiotics are alive, non-pathogenic microorganisms, which when administered in adequate amounts, reach the intestines in sufficient numbers to confer health benefits to the host [1]
The properly diluted samples were incubated anaerobically for 48 h at 37◦C and the small bacterial colony with entire margin, creamy white shiny color, convex elevation, and opaque opacity were preliminarily screened as Lactobacillus spp
The results revealed that L. oris retained around 80% viability for up to 4 h at pH 1 and 2
Summary
Probiotics are alive, non-pathogenic microorganisms (bacteria or yeasts), which when administered in adequate amounts, reach the intestines in sufficient numbers to confer health benefits to the host [1]. Before being employed as probiotics, microorganisms must have been granted Generally Regarded as Safe (GRAS) status. They must have a number of good technological properties such as simple propagation, incorporation into foods, long-term survival, safety in food products, and have clinically validated health effects [1, 4]. The majority of probiotics are lactic acid bacteria such as Lactobacillus and Bifidobacterium species that are part of human and animal intestinal flora [5, 6]. Lactobacillus species from Mother’s milk deserve significant attention, to date, very little information has been reported about probiotics from human breast milk [9,10,11]
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