Abstract
Abstract Sanger’s application of partition chromatography to the analysis of insulin was crucial in showing that different proteins have a defined sequence of amino acids arranged in a specific order. But thanks to its application by Erwin Chargaff, Martin’s and Synge’s method was also central in explaining how DNA carried genetic information. Using partition chromatography, Chargaff found striking differences in the relative amounts of nucleotide bases from nucleic acids in different species. This offered the first hint that genetic information might reside at the level of the sequence of bases. Chargaff’s analysis also played a crucial role in Watson’s and Crick’s double-helical model of DNA, first published in 1953, after which a number of scientists (George Gamow, Francois Jacob, Jacques Monod, Marshall Nirenberg) showed how the sequence of nucleotide bases in DNA determined the precise order of amino acids in a protein.
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