Abstract
Citrate uptake in Lactococcus lactis ssp. lactis biovar diacetylactis (L. diacetylactis) is catalysed by a secondary citrate carrier, the citrate permease P (CitP). In this report the mechanism of citrate uptake was investigated in resting cells of L. diacetylactis. The results presented reveal that the pH gradient (ΔpH) and the membrane potential (ΔΨ) are driving forces for citrate uptake. Moreover, it seems that CitP activity requires neither Na+ nor Mg2+ cations.
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