Abstract
NFECTIOUS bronchitis (IB) is an acute and highly infectious respiratory disease of chickens. Economic losses are due to mortality and retardation of growth in young chicks and loss of production and egg quality in layers. Shalk and Hawn first described the disease and Bushnell and Brandly2 demonstrated its viral etiology. Beaudette and Hudsonl propagated the virus in embryonated eggs and Jungherr and Terrell7 demonstrated parental immunity in chicks. Jungherr et al.8 showed the existence of two antigenically different types of IB virus. Fahey and Crawley5 propagated IB virus in chicken-embryo tissues and monkeykidney cells but could not demonstrate a cytopathogenic effect (CPE). Buthala and Mathews3 could not show any CPE on a single passage of IB virus in chicken-embryo-kidney cells. In this work the growth of IBV virus in tissue culture was investigated by using serial blind passages in an attempt to produce CPE. This characteristic of the virus would be an added tool in the study of the virus.
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