Abstract
Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC.
Highlights
Traditional Chinese medicine (TCM) has been praised in the world of medicine due to its effects in promoting cell proliferation, regulating bone metabolism, etc.[3,4]
We have obtained the following results: BER could bind to epidermal growth factor receptors (EGFR) on the cell membrane of hPDLSC; the intracellular ERK signalling pathway was subsequently activated; the nuclear-related genes FOS up-regulated; and the osteogenesis effect of hPDLSC accelerated (Fig. 6)
TCM has a variety of biological effects, such as promoting cytoplasmic proliferative activity and osteogenesis, so it is very suitable for chronic periodontitis – a chronic disease caused by bacteria
Summary
Traditional Chinese medicine (TCM) has been praised in the world of medicine due to its effects in promoting cell proliferation, regulating bone metabolism, etc.[3,4]. Because hPDLSC used in our study were primary cultured from PDL, there were a variety of membrane receptors existing in the surfaces of cells It was not clear which cell membrane receptor was bound with the drug ligand from Coptidis Rhizoma and BER and the correlation effect between BER and hPDLSC and its related signal pathway has not been reported. We propose the following hypothesis: that BER may bind to a specific receptor on the surface of the cell membrane of hPDLSC so the intracellular signalling pathway is subsequently activated, the nuclear-related genes changed until the osteogenesis effect of hPDLSC is regulated. Through the method of cell membrane activity screening, we attempted to find the target sites for BER binding to hPDLSC and the related mechanism to promote osteogenesis, in order to provide an experimental basis for the development of TCM for the treatment of periodontal bone destruction
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
