The promoting effect of ultrasound targeted microbubble destruction on establishment of type I diabetic nephropathy in rats

Abstract

Objective To explore the feasibility of the promoting effect of ultrasound targeted microbubble destruction(UTMD) on establishment of type Ⅰ diabetic nephropathy in rats. Methods Forty male SD rats were randomly and equally divided into four groups(n=10): control group(group A), streptozocin group(group B), streptozocin and ultrasound microbubble group(group C) and streptozocin and UTMD group(group D). The fasting blood-glucose (FBG) were tested, the 24 h′s urine were collected and the 24 h′s urine microalbumin(mAlb) were measured and then urinary albumin excretion rate(UAER) were calculated twice a week at fixed time in all groups. When greater than 3-fold increase in UAER compared with controls at the same age and gender, the diabetic nephropathy model in rats was considered to be established successfully, then the change of time and weighed were recorded and rats were killed and collected the blood of left atrial appendage to measure, blood urea nitrogen (BUN), serum creatinine (SCr) and Alanine aminotransferase(ALT). The left kidneys were weighted and then observed glomerular pathological changes under light microscope and detected the expression of CD34 in kidney of rats in each group by immunohistochemical method. Results ①The time of establishing diabetic nephropathy model in group D was obviously shorter than that in group B and C(all P 0.05). ②The pathological changes of diabetic nephropathy appeared in rats of group B, C and D; the expression of CD34 in B, C and D groups were raised. Conclusions UTMD can obviously shorten the molding time of type I diabetic nephropathy rats, which has feasibility. Key words: Microbubbles, target; Diabetes mellitus, type 1; Diabetic nephropathies; Rat