Abstract
Urokinase‐induced fibrinogenolysis was monitored with thrombin clotting time, and inhibited by adding Soybean Trypsin Inhibitor (STI). Depending on the concentration of STI (dialysed or non‐dialysed), the thrombin clotting time was always immediately prolonged upon the addition of STI. At a concentration of 0.2–0.5 mg/ml (final conc.) no progressive prolongation of the thrombin clotting time occurred, indicating inhibited fibrinogenolysis.By monitoring a mixture of purified human fibrinogen, thrombin and various amounts of STI, with quantitative N‐terminal analysis, the thrombin clotting time prolonging effect of STI was further examined. N‐terminal glycine increase, reflecting fibrin formation rate by thrombin, was unaffected by STI, whereas visible gelation occurred more slowly in the presence of STI.Thus, STI (or a non‐dialysable contaminant) acts as a fibrin polymerization inhibitor, and not as an anti‐thrombin, even at concentrations regularly used to inhibit fibrinogenolysis in plasma.
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