Abstract
目的 研究骨髓基质干细胞(BMSCs)与IKVAV多肽纳米纤维凝胶复合的细胞假体应用于脊髓损伤治疗的可行性.方法 合成IKVAV多肽两亲性分子,进行自组装,用透射电镜检测.将IKVAV多肽纳米纤维凝胶与BMSCs复合培养,采用倒置显微镜下观察、Calcein-AM/PI染色、CCK-8法、免疫荧光双标法,检测IKVAV多肽对BMSCs增殖、黏附及向神经细胞方向诱导分化的影响.结果 IKVAV多肽可成功自组装成纳米纤维凝胶,其与BMSCs复合培养细胞生长良好,活细胞数达90%以上,IKVAV多肽对BMSCs增殖没有影响,可促进BMSCs的黏附,并在诱导BMSCs向神经细胞分化过程中提高神经元分化比例.结论 BMSCs在IKVAV多肽纳米纤维凝胶材料表面可良好的增殖及黏附,并可提高其向神经细胞分化过程中神经元的分化比例。
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