Abstract
We have previously shown that two DNase I-hypersensitive sites are present upstream of the PRL gene in pituitary tumors of Fischer 344 rats. In this paper we present a method for examining hypersensitive sites in nontumorous pituitaries where PRL-producing lactotrophs comprise a small percentage of the total cell population. Using this method we are able to show that DNase I-hypersensitive sites in the PRL gene chromatin remain present even when estrogen is withdrawn from animals and PRL synthesis is markedly decreased. Furthermore, the hypersensitive sites appear before sexual development in female rats, and estrogen administration does not affect the appearance of the sites.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
