The Prognostic Role of m6A-Related Genes in Paediatric Neuroblastoma Patients.

Chunyang Zhang,Zhaozheng Ding,Hong Luo,Jianxin Shi

doi:10.1155/2022/8354932

Chunyang Zhang, Zhaozheng Ding + Show 2 more

Open Access

https://doi.org/10.1155/2022/8354932

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Abstract

Methods The gene expression data were extracted from the “Therapeutically Applicable Research to Generate Effective Treatments” (TARGET) database. The differentially expressed genes (DEGs) were identified, and the relationships between DEGs and m6A genes were explored. Then, the correlations among the m6A genes in neuroblastoma were investigated. Finally, the prognostic role of the m6A genes was studied, and the risk model was constructed. Results 81 NB patients were extracted from the TARGET database. After comparing the gene expression between unfavorable and favorable outcome groups, 73 DEGs were identified, including 54 downregulated genes and 19 upregulated genes. In NB patients, we found that IGF2BP3, METTL14, and METTL16 are prognostic factors for disease-free survival (DFS) while IGF2BP3, METTL14, and METTL16 are prognostic factors for overall survival (OS). Besides, after the risk model construction, the OS between the two risk groups was drawn (log-rank p = 1.64e − 08, HR = 3.438, 95% CI 2.24-5.278). The 1-, 3-, and 5-year time-dependent receiving operating characteristic (ROC) curves were also illustrated, and the areas under the receiver operating characteristic curves (AUCs) attained 0.75, 0.798, and 0.768, respectively. Conclusions IGF2BP3, METTL14, and METTL16 were identified as the significant factors for DFS and OS in NB patients.

Highlights

Neuroblastoma (NB) is the most common extracranial solid tumor in children, which originated from sympathetic ganglia and bilateral adrenal glands, and has the highest incidence rate and mortality rate in infancy, accounting for 8%-10% of children’s tumors [1]
In NB patients, we found that IGF2BP3, METTL14, and METTL16 are prognostic factors for disease-free survival (DFS) while IGF2BP3, METTL14, and METTL16 are prognostic factors for overall survival (OS)
As for survival analysis, in all the NB patients, we found thatIGF2BP3, METTL14, and METTL16 are prognostic factors for DFS (Figure 6) while IGF2BP3, METTL14, and METTL16 are prognostic factors for OS (Figure 7)

Summary

Introduction

Neuroblastoma (NB) is the most common extracranial solid tumor in children, which originated from sympathetic ganglia and bilateral adrenal glands, and has the highest incidence rate and mortality rate in infancy, accounting for 8%-10% of children’s tumors [1]. The average age at the time of clinical diagnosis is 17.3 months, and 40% of children are diagnosed before 1 year old [2]. In terms of survival rate, 85% to 90% of low- and moderate-risk children can be cured, while the survival rate of high-risk NB children is less than 50% [3]. High-risk children with NB are still difficult to cure after repeated intensive treatments. The role of m6A-related genes in neuroblastoma (NB) is unclear. This study attempted to determine the prognostic role of m6A-related genes in NB patients. The gene expression data were extracted from the “Therapeutically Applicable Research to Generate Effective Treatments” (TARGET) database. The prognostic role of the m6A genes was studied, and the risk model was constructed. IGF2BP3, METTL14, and METTL16 were identified as the significant factors for DFS and OS in NB patients

Methods

Results

Conclusion