Abstract
Purpose: To compare the Lipid peroxidation and Total antioxidant status in women with gestational diabetes mellitus and normal pregnancy in our environment. Materials & Methods: This was a 2-year, cross sectional, case control study of 25 gestational diabetes mellitus (GDM) and 75 matched normal pregnant women at Ladoke Akintola University of Technology (LAUTECH) Teaching Hospital, Osogbo, Nigeria. The study protocol was reviewed and approved by our Institutional Ethical Review Committee and all participants gave their consents. The fasting serum of recruited patients were analysed for lipid peroxidetion product malondialdehyde(MDA), based on MDA reaction with thiobarbituric acid (TBA), with rapid, sensitive and specific Thiobarbituric Acid (TBA) assay, while the Total Anti-oxidant status (TAS) was determined using the capacity of the biological fluids to inhibit the production of thiobarbituric acid reactive substances (TBARS) from sodium benzoate under the influence of the free oxygen radicals derived from Fenton’s reaction. Results: The mean serum MDA was significantly higher in the GDM group (3.64 nmol/mL) than the value recorded (1.33 nmol/ mL) in the control group (P vs. 0.66 nmol/mL) was noticed in the GDM group (P
Highlights
The World Health Organisation defined gestational diabetes mellitus (GDM) as carbohydrate intolerance resulting in hyperglycaemia of variable severity with onset or first recognition that usually during the second half of pregnancy
The fasting serum of recruited patients were analysed for lipid peroxidetion product malondialdehyde(MDA), based on MDA reaction with thiobarbituric acid (TBA), with rapid, sensitive and specific Thiobarbituric Acid (TBA) assay, while the Total Anti-oxidant status (TAS) was determined using the capacity of the biological fluids to inhibit the production of thiobarbituric acid reactive substances (TBARS) from sodium benzoate under the influence of the free oxygen radicals derived from Fenton’s reaction
A total of 100 patients were recruited for study, of which 25 were women diagnosed with gestational diabetes mellitus and 75 were normal pregnant women (Controls)
Summary
The World Health Organisation defined gestational diabetes mellitus (GDM) as carbohydrate intolerance resulting in hyperglycaemia of variable severity with onset or first recognition that usually during the second half of pregnancy. It affects 3% - 10% of pregnancies, depending on the population studied [1]. Pregnancy is a physiological condition characterised by various potentially diabetogenic hormonal changes due to increase in glucocorticoids, human placental lactogen and oestrogens This can result in insulin resistance and it may be the first life challenge of a woman’s ability to respond to a physiological stress and to detect those at greater risk of developing diabetes in the future. Oxidative stress which is a general term used to describe the steady state level of oxidative damage to cell, tissue or organ, caused by the elevated reactive oxygen species (ROS) [4], has been associated with type 2 diabetes mel-
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
