The profile and percentage of vaginal epithelial cell numbers during the estrous cycle in Bali cattle

Abstract

The aim of this study was to observe the percentage of the number and the diameter of vaginal epithelial cells during the estrous cycle in Bali cattle. Six female Bali cattle were used in this research. Sample collection and estrous detection were observed at 06.00-07.00 am and 17.00-18.00 pm for 28 days. The method of this research used vaginal smear with Giemsa staining and then observed by microscope. Image Raster 3.0 was used to measured cells diameter and data were analysed using Independent T-Test. Based on the results of this study, the vaginal epithelial cells during the estrous cycle were intermediate cells, parabasal cells, superficial cells, and cornification cells. The percentage of intermediate cell numbers increased in the proestrus phase and decrease in the estrous phase. The percentage of parabasal cell numbers increased in metestrus and diestrus phase along with decreased in proestrus phase and not found in the estrous phase. The percentage of superficial and cornification cell numbers increased in the estrous phase and last stage of the proestrus phase along with decreased in the metestrus phase and not found in the diestrus phase. In measuring the diameter of the vaginal epithelial cells, superficial cells have a larger cytoplasmic diameter and the smallest diameter of the cytoplasm was parabasal cells. Whereas in measuring the nucleus diameter and diameter ratio of the cytoplasmic nucleus, parabasal cells have a larger diameter and cornification cells have smallest nucleus diameter and cytoplasmic nucleus diameter ratio.