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Abstract
Tissue culture techniques were used to prepare high titers of Newcastle disease virus labeled with P 32. Techniques for purifying this radioactive virus are presented, and criteria for purity are described. The purified virus was fractionated by the Schmidt-Thannhauser procedure to determine the chemical distribution of P 32. The chemical components were investigated in some detail and the results indicate that while some of the small molecular phosphorus containing material in the TCA soluble fraction may not be essential to the virus, the phospholipid fraction is essential. P 32 was consistently found in the Schmidt-Thannhauser ribonucleic acid (RNA) fraction, while very little appeared in the deoxyribonucleic acid (DNA) fraction, suggesting that the virus contains little or no DNA.
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