Abstract
In bacteria which exhibit a tendency to the formation of ‘cytoplasmic granules’, similar granules can be produced artificially by the adsorbtion of DNA of extraneous origin and staining with a modified Feulgen reaction. These granules appear mainly in the surface cytoplasm and cell envelopes, especially in the septa, and may simulate true stuctures. This phenomenon supports the suggestion that differences in nuclear and cytoplasmic structures in bacteria subjected to various cytological procedures may be due to transfer of basophilic materials between different parts of the cell. It also emphasises the need for extreme caution in interpreting such minute granular structures, in respect both of their morphology and cytochemistry.
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