Abstract
ABSTRACT The production of essential oil from Pelargonium graveolens by plant tissue culture was investigated. Since the essential oil could not be obtained by callus culture, shoot culture was investigated. Sterile plants were induced from intact plants by using solid medium with appropriate hormones. Young shoots which also were obtained from sterile plants, were well cultivated in liquid medium by a method of agitating with air. The odor of the essential oil obtained from cultured shoots was found to be similar to that of the natural essential oil. Furthermore, the GC pattern of the volatile compounds in the essential oil from cultured shoots was similar to that of natural essential oil. The content of citronellol, a major constituent of natural Pelargonium leaf oil, was slightly less in the cultured shoots than in the intact plant.
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