Abstract
Using an in vitro system for tail attachment, it is demonstrated that following ultraviolet (UV) induction of Salmonella typhimurium LT2 (P22) most of the particles formed lack a functional tail. This effect is found at all temperatures studied, but is more pronounced at temperatures of 37° and above. Similar results are described for thermal and spontaneous induction. Tailless particles are found following infection only at temperatures of 42° and above. Temperature pulse experiments on UV-induced lysogens suggest that late in the growth cycle tail synthesis is inhibited by high temperature. There is also an earlier temperature-sensitive step, which probably causes the production of inactive heads at 40°.
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