Abstract
The method of glucose oxidase production (β- d-glucose: oxygen 1-oxidoreductase, E.C. 1.1.3.4, GOD) using the waste myceliums of Aspergillus niger is studied. The results show that the waste mycelium from the production of NaG, KG, CaG 2, and MgG 2 (G = gluconate) can be used to produce the GOD. The effects of metal ions on the GOD activity are also studied. At lower salt concentrations, metal chlorides and metal gluconates activate the GOD activity. At higher salt concentrations, the metal chlorides and metal gluconates show various degrees of inhibition. At the same salt concentration, the inhibition of metal chlorides is stronger than that of metal gluconates.
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