Abstract
The synthesis of cysteinesulfinic acid from cysteine by the 105000 × g supernatant of rat liver is described. A method for 5- to 10-fold purification of the activity is presented. Properties of the activity according to cysteine and cysteinesulfinic acid concentrations, pH, time of incubation and use of cystine as a substrate are examined. The variation of cysteinesulfinic acid production by individual rat livers is demonstrated and shown to be related to the metabolism of cysteinesulfinic acid. Activity is demonstrated only in the 105000 × g supernatant of liver and cannot be shown to be present in any fraction (or combination of fractions) of heart, kidney, spleen or brain.
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