Abstract
Purified oligodendroglia maintained in culture produce whorls of membrane lamellae, adjacent to the cell soma. When subcellular fractions are prepared from the cells in culture, three membrane fractions are obtained: a glial light fraction which electron micrographs show to be predominantly large vesicles; an intermediate fraction that on electron micrographs consists of whorls of membrane lamellae; and a plasma membrane fraction consisting primarily of small vesicles. In a study on the production of the membrane lamellae, the results show that there is a rapid incorporation of radiolabel into cerebrosides and phosphatidylcholine, with lower incorporation into other phospholipids. There is a delay in incorporation into sulfatides. Incorporation into proteins show a complex heterogeneous pattern of proteins, ranging from high to low molecular weight (MW) bands. The incorporation data may reflect the composition of the subfractions after different times in culture.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
