Abstract

High yields of influenza A/PR8, comparable to those obtained in the 10-day chick embryo, were obtained using a low input multiplicity of infection in suspension cultures of a continuous line of mammalian cells (G2). By using the hemadsorption differential (HAD) count, a technique combining hemadsorption with viable cell counting, the progress of virus production can be assayed and correlated with the metabolic condition of the cells.

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