Abstract
The possibilities for autoradiographic detection of 51Cr-labelled lymphocytes were studied. It appeared that the bulk of the 51Cr was not fixed in the cells after fixation and cell death. This was concluded from the high background in the autoradiographs, caused by 51Cr eluted from the cells, and from the varying labelling results which were obtained. Due either to the 51Cr elution from the cells or to participation of gamma-rays in the autoradiographic results or both, grains were also formed at a distance of several microns from the 51Cr labelled cells.
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