Abstract
Most recent developments in fluorescent molecular sensor devices are based on "ON-OFF" switching, which is an operation with a single measurable parameter, commonly the quenching of total intensity. In the meantime, with this approach self-calibration on the molecular level is not achievable. This calibration is strongly needed in all kinds of microscale applications, including microarrays, microfluidic systems and living cell imaging. Different possibilities are discussed for overcoming this difficulty and an "OR-OR" switching concept is suggested that involves the two-channel detection as a promising solution. For achieving the desired efficiency specific conditions are needed: it should be a single reporter dye exhibiting rapid reversible excited-state reaction and providing two-band wavelength ratiometric response.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
