Abstract
Murine cytomegalovirus (MCMV) infection in mouse fibroblasts resulted in an early inhibition in thymidine incorporation into cell DNA. In contrast [32P]phosphate incorporation was only slightly inhibited, while the incorporation of deoxycytidine, deoxyadenosine, and deoxyguanosine continued unabated. The limiting factor in thymidine incorporation was a decrease in uptake by the infected cells. Uptake of the other nucleosides, however, was either normal or enhanced. Nuclear monolayers, derived from infected cells, incorporated thymidine triphosphate into cell DNA at the same rate as nuclei of uninfected cells. Measurement of total DNA content of the cells revealed no difference between infected and control cultures. Thus MCMV neither inhibits nor stimulates cellular DNA synthesis.
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