THE PROBLEM OF CELL CULTURES CONTAMINATION WITH MAMMALIAN ORTHOREOVIRUSES

E B Faisuloev,D V Markov,O A Petrusha,V V Zverev,E P Korchevaya

doi:10.36233/0372-9311-2018-5-104-107

Abstract

A mandatory requirement for cell cultures used in scientific researches and biomanufacturing is the absence of their contamination by viruses. We have described the case of adventitious isolation of mammalian orthoreovirus in the rhesus macaque embryo kidney cells MA-104. PCR analysis for the presence of reovirus RNA of all probable sources of reovirus (trypsin, fetal bovine serum, clinical samples, cell culture) revealed no viral RNA in any of the samples. An important condition for the activation of the reovirus reproduction in the MA-104 cells was the presence of trypsin in the culture medium. The obtained results underscore the urgency of control for the reovirus contamination of chemicals of animal origin and cell cultures. Since reoviruses are associated with human diseases, such control in pharmaceutical production is mandatory.

Highlights

11.Develioglu O.N., Ipek H.D., Bahar H. et al Bacteriological evaluation of tonsillar microbial flora according to age and tonsillar size in recurrent tonsillitis
12.Kuhn J.J., Brook I., Waters C.L. et al Quantitative bacteriology of tonsils removed from children with tonsillitis hypertrophy and recurrent tonsillitis with and without hypertrophy
We have described the case of adventitious isolation of mammalian orthoreovirus in the rhesus macaque embryo kidney cells MA-104

Summary

Introduction

Обязательным требованием к культурам клеток, используемым в научных исследованиях и биотехнологическом производстве, является отсутствие их контаминации вирусами. Нами описан случай незапланированного выделения в культуре клеток почки эмбриона макаки-резуса МА-104 ортореовируса млекопитающих. ПЦР-анализ на наличие реовирусной РНК всех вероятных источников реовируса (трипсин, эмбриональная сыворотка коров, клинические образцы, культура клеток) не выявил вирусной РНК ни в одном из образцов. Важным условием активации репродукции реовируса в культуре клеток МА-104 являлось наличие в культуральной среде трипсина.

Results

Conclusion