Abstract
The complete amino acid sequence of cytosolic serine hydroxymethyltransferase from rabbit liver was determined. The sequence was determined from analysis of peptides isolated from tryptic and cyanogen bromide cleavages of the enzyme. Special procedures were used to isolate and sequence the C-terminal and blocked N-terminal peptides. Each of the four identical subunits of the enzyme consists of 483 residues. The sequence could be easily aligned with the sequence of Escherichia coli serine hydroxymethyltransferase. The primary structural homology between the rabbit and E. coli enzymes is about 42%. The importance of the primary and predicted secondary structural homology between the two enzymes is discussed.
Highlights
The complete amino acid sequence of cytosolic serine hydroxymethyltransferase from rabbit liver was determined
The sequence could be aligned with the sequence of Escherichia coli serine hydroxymethyltransferase.The primary structural homology between the rabbit and E. coli enzymes is about 42%.The importance of the primary and predicted secondary structural homology between the two enzymes is discussed
In this paper we report the complete amino acid sequence of rabbit liver cytosolic serine hydroxymethyltransferase which has been determined by classical protein sequencing methods
Summary
The amino acid sequence of rabbit liver cytosolic serine hydroxymethyltransferase is compared in Fig. 2 with that of the protein codedby the glyA gene of E. coli, whichwas identified as the bacterial version of this enzyme [10]. We have recently changed an active site histidine to an asparagine in the E. coli enzyme [2] The importance of this histidine was suggestedby the conservation of an 11-residue amino acid sequence between the rabbit cytosolic and mitochondrial isozymes and theE. We are currently sequencing these cDNA clones to see whether they code for cytosolic serine hydroxymethyltransferase
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
