The Primary Structure of Alanine Transfer Ribonucleic Acid 1 from <italic>Torulopsis utilis</italic><subtitle>II. Partial Digestion with Ribonuclease T<sub>1</sub> and Derivation of the Complete Sequence</subtitle>

Abstract

Partial RNase T1[EC 2.7.7. 26] digestion of tRNAAla1(Torulopsis utilis) in the presence of Mg2+ yielded two half molecules by scission at the anticodon site. Several large oligonucleotides smaller than the halves were obtained by partial RNase T1 digestion of the tRNA in the absence of Mg2+. The sequences of these large fragments were analyzed. The complete sequence of this tRNA was derived from the results of these analyses and those described in the preceding paper. The cloverleaf model for the secondary structure was compared with that of Saccharomyces tRNAAla, as regards the aminoacyl-tRNA synthetase recognition sites.