The prevention of single bulb garlic extract on non-alcoholic fatty liver disease (NAFLD) in high fat mice (Mus musculus) model

Abstract

Non-alcoholic fatty liver disease (NAFLD) occurs when triglycerides inside hepatocytes are swollen. A single bulb garlic extract containing allicin components, S-allyl-l-cysteine, Ajoene (E-Ajoene and Z-Ajoene) are thought to inhibit fat biosynthesis and reduce triglycerides in blood serum. This study aimed to analyze single bulb garlic extract in NAFLD due to a high-fat diet (HFD) in mice. Twenty male Balb/C mice were divided into five groups: normal, HFD, HFD + Single Bulb Garlic Extract (SBGE) 100 mg kg BW−1, HFD + SBGE 200 mg kg BW−1, and HFD + SBGE 400 mg kg BW−1. The administration of SBGE orally altogether with HFD for 30 days. On the last day of treatment, the mice were sacrificed. The liver was collected to make a micro-anatomic slide and stained by Hematoxylin Eosin. Liver microanatomy slides were observed under a light microscope and scoring to count fatty liver cells. The results of this study indicated that administration of SBGE affected the average liver weight (p < 0.05). Treating SBGE with dose of 100 mg kg BW−1, 200 mg kg BW−1, and 400 mg kg BW−1 decreased hepatic steatosis (p < 0.05). SBGE could prevent non-alcoholic fatty liver disease in mice fed with HFD.