Abstract
Purpose:To examine selected putative virulent prop-erties of mutans streptococci in two groups with dif-ferent caries activity and to examine co-culture hy-bridization of the strains in vitro. Methods: A set of strains from caries-free subjects (115) and another set from caries-active subjects (165) were isolated. Each strain was characterized for three virulence determi-nants. The clinical bacteria were then cocultured by three strains exhibiting the highest levels of virulence. Isolate colonies of last filial generation bacteria were enrichment-incubated and estimated for virulence again. RAPD-PCR and MLEE analyses were processed for parental bacteria and last filial genera-tion one. Results: No difference associated with caries activity of the subjects from whom the isolates origi-nated. Virulent properties of a filial generation strains was not different in the same generation, but was very different from their parent strains. Conclu-sion: The coexist properties of virulent polyclonal strain of MS may hold in a very general conditional sense in a dental plaque ecosystem in vivo, however, one of the co-culture strains may became dominant and displa- ced the others as the result of continuous ecological succession in vitro.
Highlights
Dental caries is a transmissible and poly-microbial ecological disease, in which mutans streptococci (MS), mainly Streptococcus mutans and Streptococcus sobrinus play the major role
A total of 26 of 96 strain colonies from last filial generation were subjected to RAPD-PCR analysis
RAPD-PCR data showed that the band profiles of filial generation bacteria were not significantly different from each other but different from any ones of the parent strains (Figure 2)
Summary
Dental caries is a transmissible and poly-microbial ecological disease, in which mutans streptococci (MS), mainly Streptococcus mutans and Streptococcus sobrinus play the major role. The main virulent factors of MS include adhesion, acidogenicity and aciduricity [1,2,3]. Acid production and aciduricity are other important caries-inducing virulent factors [6,7,8]. MS is generally considered to be the principal aetiological agent of dental caries [9,10]; they are widely distributed in populations with moderate or high caries prevalence [11,12] and in populations having no or low caries experience [13,14]. Caries can develop in the absence of MS species [15]
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