The Prevalence of Inducible Clindamycin Resistance Staphylococcus aureus among Various Clinical Specimens in Khartoum state Sudan

Abstract

Background : S. aureus is frequently associated with skin infections, pneumonia, surgery wounds, bacteraemia, osteomyelitis and endocarditis, being considered one of the most important pathogens of the human being, both at the community level and at nosocomial infections, and may become serious if caused by antimicrobial resistant strains, especially methicillin-resistant S. aureus (MRSA) strains, which are resistant to most of the antimicrobial agents, methicillin-sensitive S.aureus (MSSA) and isolates with reduced susceptibility and resistance to vancomycin, which is the last drug for the treatment of MRSA infections. So, this study aimed to detect the existence of inducible resistance of S. aureus to Clindamycin in Khartoum-Sudan among patients attended to Suba University Hospital. Methods : The study was performed as cross-sectional one, 53 clinical isolates of S. aureus obtained from (34 females and 19 males) with different clinical condition among patients attended to Suba University Hospital in Khartoum-Sudan from April to August 2017. To detect inducible clindamycin resistant by using D test. In addition to that MRSA / MSSA all the isolates screened for methicillin resistant by using 1 µg oxacillin then examined for inducible clindamycin resistant by D test. In addition to that examine for antimicrobial susceptibility profile which include vancomycin, gentamycin, tetracycline and co-trimoxazole. The data were analyzed using Statistical Package for Social Science, version 22, P. value <0.05 was considered statistically significant Results : out of 53 isolates, 36 S. aureus isolated resistant to Clindamycin, 26 (72.2%) were MRSA and 10 (27.8%) were MSSA by means of D test, while 17 (32.1%) of isolates were sensitive 9 (53%) MRSA and 8 (47 %) MSSA. Comparing Induced clindamycin resistance showed equally distribution among MSSA and MRSA isolates, giving no significant difference as P- value 0.167. Conclusion : This study showed that D.test to detect inducible clundamycin resistance in staphylococcus aureus.