The prevalence of human papillomavirus types 52 and 58 in uterine cervical lesions from Japanese women.

Abstract

1543 Background: In recent years, molecular biology has firmly established a causal relationship between persistent infection with high-risk human papillomavirus (HPV) genotypes and cervical cancer. While HPV 16 is the most prevalent type in the world, it has been reported that the frequency of other high-risk HPV types varies by region. We estimate the prevalence and genotypes of high-risk HPV focusing HPV 16, 18, 52, and 58, using liquid-base cytology (LBC) in Japan. Methods: ThinPrep LBC specimens were collected from Japanese women (n=11022), aged 14-98. After classifying cytodiagnosis by the Bethesda system 2001, specimens were analyzed for HPV DNA by the multiplex polymerase chain reaction method (PapiPlexTM), where 1195 specimens were positive for cervical smear except adenomatous lesions and 2068 samples were randomly selected from 9760 did not display abnormal cytological findings (NILM). Written informed consent was obtained from all patients. Results: Atypical squamous cells of undetermined significance (ASC-US) or severer cytological findings were found in 1195 (10.8%, 1195/11022) cervical smears. HPV genotypes were detected in 9.5% (194/2068) of NILM and 72.2% (841/1195) of ASC-US or more cervical lesions. In NILM, HPV genotypes and positive rates were HPV 52 (23.7%, 46/194), HPV 58 (17.0%, 133/194), HPV 16 (15.5%, 30/194), and HPV 18 (10.8%, 21/194). In positive cervical smears, HPV genotypes were HPV 52 at 26.6%, HPV 16 at 25.2%, HPV 58 at 21.8%, and HPV 18 at 7.1%. Most patients infected with HPV 16 were between 20-29 years old, decreasing with age thereafter. As for HPV 52 and 58, although the detection rate was high in 30- to 39-year-olds, it also was significant in 50s and 60s age groups. Conclusions: In Japan, as a cause of abnormal cervical cytology, HPV52 and 58 are detected frequently in addition to HPV 16. In older age groups, HPV 52 and 58 were higher than that observed for HPV 16. After widespread current HPV vaccination, we still must be aware of HPV 52 and 58 infections.