Abstract
Background and Objective:Blood-borne infections, such as the HIV virus and hepatitis B and C, are major problems in patients receiving blood products. Here we examined the prevalence of HTLV-1, HCV, HBV, and HIV in hemophilic patients.Methods:A cross-sectional study on 108 hemophilic patients (101 males and 7 females) involved detection of HBV, HCV, HIV and HTLV-1 infections using immunoassays for HBsAg, hepatitis B core antibodies (anti-HBc), hepatitis C antibodies (anti-HCV), HIV antibodies (anti-HIV) and Anti-HTLV-1. Real-time PCR was used to measure HCV RNA, and HCV genotyping was performed by direct sequencing of the 5’ noncoding region.Results:Hemophilia A was reported in 93 (86%) patients with severe symptoms in 8 cases. The seroprevalence of anti-HCV and anti-HTLV-1 antibodies was 20% and 3% respectively. One patient with severe hemophilia had a HCV/HTLV-1 co-infection. HCV-RNA was detected in 82% of patients. In terms of genotyping prevalence was 56% HCV genotype 3a, 39% HCV genotype 1a, and 6% HCV genotype2. Anti HIV and HBsAg were not detected in any patient. HTLV1 prevalence was higher, HCV lower in South Khorasan than other regions in Iran or elsewhere.Conclusion:Management of transfusion of blood and blood products should account for the underlying prevalence of infectious agents.
Highlights
Hemophilia is a disease characterized by serious bleeding symptoms due to a deficiency of factor VIII for hemophilia A and factor IX for hemophilia B (Christmas disease).[1]
The seroprevalence of anti-hepatitis C virus (HCV) antibody was 20.4% (22 of the 108 patients); whereas 18 (82%) of these 22 patients who tested positive with anti-HCV had positive results in the RT-PCR method. Among these 18 positive results, HCV genotype was determined and there were 10 (55.5%) patients infected with genotype 3a, seven patients (39%) with genotype 1a, and one patient (5.5%) with genotype 2a
According to sequencing of 5’ noncoding region in HCV positive samples, there were 10 (55.5%) patients infected with genotype 3a, 7 (39%) with genotype 1a and 1 (5.5%) with genotype 2a
Summary
Hemophilia is a disease characterized by serious bleeding symptoms due to a deficiency of factor VIII for hemophilia A and factor IX for hemophilia B (Christmas disease).[1]. Blood-borne infections, such as the HIV virus and hepatitis B and C, are major problems in patients receiving blood products. We examined the prevalence of HTLV-1, HCV, HBV, and HIV in hemophilic patients. Methods: A cross-sectional study on 108 hemophilic patients (101 males and 7 females) involved detection of HBV, HCV, HIV and HTLV-1 infections using immunoassays for HBsAg, hepatitis B core antibodies (antiHBc), hepatitis C antibodies (anti-HCV), HIV antibodies (anti-HIV) and Anti-HTLV-1. One patient with severe hemophilia had a HCV/HTLV-1 co-infection. HCV-RNA was detected in 82% of patients. Anti HIV and HBsAg were not detected in any patient. HTLV1 prevalence was higher, HCV lower in South Khorasan than other regions in Iran or elsewhere. Conclusion: Management of transfusion of blood and blood products should account for the underlying prevalence of infectious agents
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