The prevalence of extended spectrum bêta-lactamase-producing Escherichia coli isolated in wastewater in relation to patients hospitalized in the city of Meknes in Morocco

Khadija Ouarrak,Rachid Sammoudi,Adel Kharroubi,Hajar El Omari,Aziz Taouraout,Abdelkader Chahlaoui,Imane Taha,S Bourekkadi,H Hami,K Slimani,A Mokhtari,A Soulaymani

doi:10.1051/e3sconf/202131902014

Abstract

The present study was conducted to better understand the specific contingency of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) isolated from urban waters of Wadi Ouislane of the city of Meknes, compared to isolates from patients hospitalized in the resuscitation department at Mohamed V Hospital in Meknes, Morocco. These antibiotic-resistant bacteria have become ubiquitous in effluents, creating increasing concern about their potential impact on human and animal health and the environment. We took four samples of wastewater representative of a day, effluents of the wadi Ouislane. They were analyzed for indicator germs of fecal pollution, namely total coliforms (TC) and fecal coliforms (FC). Bacteria were enumerated by the dilution-filtration technique and by incorporation in solid medium in supercooling. However, four bacteriological samples, taken for clinical purposes from hospitalized patients, were performed at the medical analysis laboratory of Mohamed V Hospital in Meknes. Analysis of our results showed that ESBL-producing E. coli bacteria isolated from our effluents had the same antibiotic resistance profiles as those from hospitalized patients. Urban wastewater discharges into the environment contribute to the dissemination of extended-spectrum beta-lactamase-producing Escherichia coli that may pose health risks to the population.

Highlights

Bacterial resistance to antibiotics is a global phenomenon and the emergence of multi-resistant bacteria, in particular extended-spectrum β-lactamase (ESBL) producing enterobacteria, is a real public health problem in developing countries [1, 2]
In the current state of knowledge, the comparative study of extended-spectrum betalactamineproducing Escherichia coli isolated from surface waters of Wadi Ouislane with those identified in clinical samples (ECBU) of hospitalized patients is a first study at the national level
Phenotypic analysis revealed that station O4 contained 50% extended spectrum betalactam (ESBL) producing Escherichia coli isolates and 25% multiresistant (MR) cephalosporin-susceptible 3rd generation Escherichia coli

Summary

Introduction

Bacterial resistance to antibiotics is a global phenomenon and the emergence of multi-resistant bacteria, in particular extended-spectrum β-lactamase (ESBL) producing enterobacteria, is a real public health problem in developing countries [1, 2]. We aim to describe the antimicrobial resistance situation of Escherichia coli in urban waters of Wadi Ouislane and clinical samples with a particular focus on extended spectrum betalactam (ESBL) producing isolates and to make a comparison of the prevalence of antibiotic resistance between these two ecosystems. Together, these results should show the fate of these multidrugresistant hospital-acquired bacteria in the environment and explore the options available to control this concern. These results should show the fate of these multidrugresistant hospital-acquired bacteria in the environment and explore the options available to control this concern. 2 MATERIAL AND METHODS

Objectives

Results

Conclusion