Abstract
Background:Staphylococcus aureusis one of the most important opportunistic pathogens. Impetigo is the common contagious bacterial infection of the skin caused byS. aureus.Method:Samples were taken from 204 patients with impetigo disease.S. aureusisolates were tested for their antimicrobial susceptibility. Genomic DNA ofS. aureuswas used to transformE. coliHB101 strain and expression capability ofS. aureusplasmids in transformedE. coliwas investigated. 68.62% (140/204) of the specimens were nonbullous impetigo and 31.38% (64/204) were bullous impetigo.S. aureusstrains were isolated from 41.66% (85/204) of impetigo cases (82.35% from nonbullous and 17.65% from the bullous impetigo). There was an inverse relationship between the incidence ofS. aureusisolated and age.Result:Three biotypes ofS. aureuswere identified based on their fermentation of different sugars. All isolates were resistant to penicillin and most isolates were resistant to ampicillin (95.3%), amoxicillin, (94.11%) and cephalexin (90.95%). Most isolates were sensitive against vancomycin and rifampicin (98.83%). 5.88% (5/85) ofS. aureusisolates were identified as MRSA. A maximum of 5 markers fromS. aureusisolates were capable to be expressed in transformedE. coliHB101 strains. The incidence of impetigo caused byS. aureusis comparable with reports from elsewhere.S. aureusisolates showed multidrug resistance against antibiotics.Conclusion:Plasmids ofS. aureusare capable to show its expression inE. coliHB101. Molecular study is needed to investigate the role of plasmids in different patterns of multi drug resistance.
Highlights
Staphylococcus aureus is a major pathogen responsible for both nosocomial and community acquired infections [1]
The incidence of S. aureus isolated from nonbullous impetigo included 70 isolates (82.35%) and 15 isolates (17.65%) were from the bullous impetigo specimens
Our results were in agreement with those obtained from other studies which showed that nonbullous impetigo was the most common form of impetigo and that S. aureus was the main bacterium that causes nonbullous impetigo [4, 15]
Summary
Staphylococcus aureus is a major pathogen responsible for both nosocomial and community acquired infections [1]. The severity of these infections varied from local simple wounds to severe systemic diseases. This pathogen causes a range of diseases, including skin abscesses, wound infections, osteomyelitis, endocarditis, pneumonia, bacteremia and toxemic syndromes [2]. Impetigo is a common contagious bacterial infection of the skin caused by S. aureus This infection is mostly seen in children, especially in tropical countries [3]. Impetigo is the common contagious bacterial infection of the skin caused by S. aureus
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