Abstract
Abstract In healthy and tobacco mosaic virus (TMV)-infected tissues infiltrated with TMV, there are quantitative and qualitative differences in virus cell wall attachment, and in the retention of virus or its coat protein. An 125 I-labeled antibody technique was combined with cell wall digestion to determine antigen content of Nicotiana silvestris cell walls from light green (LG) and dark green (DG) areas of infected leaf tissues. The values (expressed in μg of bound 125 I-labeled TMV-U 1 -IgG/mg cell wall) ranged between 1 and 1.5 for light green (LG) and from 0.2 to 0.5 for dark green (DG) tissues of systemically infected leaves. Autoradiography with 125 I-labeled anti-TMV-U 1 -Fab fragments of IgG showed that Fab fragments can penetrate cell walls of tobacco leaf tissue, since viral antigen was shown to be present in cell walls of cells sustaining virus multiplication by this method.
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