Abstract
Although anti-human leukocyte antigen antibodies (anti-HLA Abs) are important factors responsible for graft rejection in solid organ transplantation and play a role in post-transfusion complications, their role in allogeneic hematopoietic stem cell transplantation (allo-HSCT) has not been finally defined. Enormous polymorphism of HLA-genes, their immunogenicity and heterogeneity of antibodies, as well as the growing number of allo-HSCTs from partially HLA-mismatched donors, increase the probability that anti-HLA antibodies could be important factors responsible for the treatment outcomes. We have examined the incidence of anti-HLA antibodies in a group of 30 allo-HSCT recipients from HLA-mismatched unrelated donors. Anti-HLA Abs were identified in sera collected before and after allo-HSCT. We have used automated DynaChip assay utilizing microchips bearing purified class I and II HLA antigens for detection of anti-HLA Abs. We have detected anit-HLA antibodies against HLA-A, B, C, DR, DQ and DP, but no donor or recipient-specific anti-HLA Abs were detected in the studied group. The preliminary results indicate that anti-HLA antibodies are present before and after allo-HSCT in HLA-mismatched recipients.
Highlights
Allogeneic hematopoietic stem cell transplantation is an effective treatment of both congenital and acquired disturbances of hematopoiesis, especially of hematological malignancies.The selection of the optimal donor is based on highresolution HLA typing
HLA mismatches may occur at antigenic or allelic level; the first are characterized by amino acid substitutions in both peptide-binding and T-cell recognition regions, whereas the latter are characterized by amino-acid substitution in the peptide binding regions only [1]
Our preliminary results indicate that preformed anti-HLA Abs can be detected before and may appear after transplant in mismatched allo-HSCT recipients
Summary
Allogeneic hematopoietic stem cell transplantation (alloHSCT) is an effective treatment of both congenital and acquired disturbances of hematopoiesis, especially of hematological malignancies.The selection of the optimal donor is based on highresolution HLA typing. Allogeneic hematopoietic stem cell transplantation (alloHSCT) is an effective treatment of both congenital and acquired disturbances of hematopoiesis, especially of hematological malignancies. The MHC (Major Histocompatibility Complex) contains more than 200 genes which are situated on the short arm of chromosome 6 at 6p21.3. It is divided into three main regions: HLA class I (containing HLA-A, B, and C genes), class II (containing HLA-DR, DQ, and DP genes), and class III region. HLA mismatches may occur at antigenic or allelic level; the first are characterized by amino acid substitutions in both peptide-binding and T-cell recognition regions, whereas the latter are characterized by amino-acid substitution in the peptide binding regions only [1]
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