Abstract
An ATPase stimulated by HCO 3 − -ions and inhibited by SCN-ions could be found in glandula submandibularis of rabbit with following properties: 1. It is stimulated by a variety of oxybases according to a general base catalytic mechanism. 2. It is inhibited by both SCN−-and OCN−-ions. 3. It can be solubilized by Triton X 100 hereby yielding a marked increase in the activity response to HCO 3 − -and SCN−-ions. 4. It can be distinguished from mitochondrial ATPase because of the different behaviour of this enzyme and mitochondrial marker such as succinic dehydrogenase using sucrose gradient centrifugations.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have