Abstract

BackgroundShenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. In order to understanding the prescription principle, the effects of “tonifying qi and strengthening spleen” group (TQSS) including Poria cocos (Schw.) Wolf. and Panax ginseng C.A.Mey and “eliminating phlegm and strengthening intelligence” group (EPSI) composed of Polygala tenuifolia Willd., Acorus calamus L. and Sinapis alba L from the herb complex on neurite growth in PC12 cells, two disassembled prescriptions derived from Shenghui soup and their molecular mechanisms were investigated.MethodsFirstly, CCK-8 kit was used to detect the impact of the two prescriptions on PC12 cell viability; and Flow cytometry was performed to measure the cell apoptosis when PC12 cells were treated with these drugs. Secondly, the effect of the two prescriptions on the differentiation of PC12 cells was observed. Finally, the mRNA and protein expression levels of GAP-43 were analyzed by RT-PCR and western blot, respectively.Results“Tonifying qi and strengthening spleen” prescription decreased cell viability in a dose-dependent manner, but had no significant effect on cell apoptosis. Meanwhile, it could improve neurite growth and elevate the mRNA and protein expression level of GAP-43. “Eliminating phlegm and strengthening intelligence” prescription also exerted the similar effects on cell viability and apoptosis. Furthermore, it could also enhance cell neurite growth, with a higher expression level of GAP-43 mRNA and protein.Conclusion“Tonifying qi and strengthening spleen” and “eliminating phlegm and strengthening intelligence” prescriptions from Shenghui soup have a positive effect on neurite growth. Their effects are related to the up-regulating expression of GAP-43.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1339-y) contains supplementary material, which is available to authorized users.

Highlights

  • Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness

  • The tonifying qi and strengthening spleen” group (TQSS) and eliminating phlegm and strengthening intelligence” group (EPSI) treatment inhibited the viability of PC12 In order to investigate the effect of the separated prescriptions (TQSS group and EPSI group) from Shenghui soup on PC12 viability, different concentrations of the two prescriptions were supplemented to the low serum media

  • In summary, the tonifying qi and strengthening spleen formula and eliminating phlegm and strengthening intelligence recipe derived from Shenghui soup can promote the extension of neurite, enhance the differentiation of PC12 cells, and increase the expression of Growth Associated Protein 43 (GAP-43)

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Summary

Introduction

Shenghui soup is a traditional Chinese herbal medicine used in clinic for the treatment of forgetfulness. Panax ginseng C.A.Mey and “eliminating phlegm and strengthening intelligence” group (EPSI) composed of Polygala tenuifolia Willd., Acorus calamus L. and Sinapis alba L from the herb complex on neurite growth in PC12 cells, two disassembled prescriptions derived from Shenghui soup and their molecular mechanisms were investigated. The ability of learning and memory is impaired in neurodegenerative disorders including Alzheimer’s disease [1], Parkinson’s disease [2], Huntington’s disease [3], etc. It. Synaptic plasticity is the neurochemical foundation of learning and memory. Synaptic plasticity is the neurochemical foundation of learning and memory It refers to the activity-dependent modification of the strength or efficacy of synaptic transmission [8]. Accumulating evidence suggests that impairments in synaptic mechanisms contribute to several prominent neuropsychiatric disorders including dementia [9] and extrapyramidal disorders [10]. The structural plasticity of synapse is dependent on various internal

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