The preparation of two bis-diazo dyes for intravenous injection.

Abstract

Summary Aqueous solutions of vital red and trypan red can be sterilised by autoclaving for 30 minutes at 10 lb. without a change in colour or an increase in toxicity. Solutions should be clarified by filtration through a No. 5/3 sintered glass filter or by centrifuging. Loss of dye can occur through incomplete solution or by adsorption to the glass filter. The concentration of the dye in the final solution should be determined colorimetrically and suitably adjusted prior to autoclaving in the final containers.