Abstract
THE PRESENT KNOWLEDGE of the chemical nature of the pituitary hormones is inadequate. In view of the importance of the hypophysis in the control of the thyroid gland, the chief objective of the present study was to develop more satisfactory methods of extraction, separation and purification of the thyrotropic hormone. Methods of preparation and purification of this hormone have been reported (1–4). In the preliminary work we attempted to evaluate quantitatively the usefulness of each of these procedures. Our experience in this regard indicates that a large percentage of the thyrotropic hormone is discarded in these methods of purification. Methods of biological assay. The analytical determination of the thyrotropic activity of the pituitary extracts was based upon the histological changes in the thyroid of the guinea pig according to the method of Junkmann and Schoeller (1) and in the thyroid of the 8 to 2-day-old White Leghorn chick.
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