Abstract
Mitomycin C, daunomycin and adriamycin, three potent cancer chemotherapeutic agents, were linked to immunoglobulins by the cyanogen bromide method and the conjugates were separated on Sepharose 6B. The most suitable conditions for binding of the drug to the antibody, while retaining both antibody and drug activity, included the use of 25-50 mol of cyanogen bromide per mol of IgG at pH 11 for the activation of immunoglobulin. The extent of substitution in this preparation was about 1 mol of drug per mol of IgG. This conjugate might be considered as a sustained release form of the antitumor agent.
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