The preparation of injected and cleared "whole-mounts" for the study of topographical anatomy.

Abstract

AbstractA simple method is described for the preparation of cleared and injected “whole‐mounts” of large masses of tissue (for example the mediastinal contents). This technique allows the use of high magnifications during examination and microphotography and the use of histological controls. It provides a novel method for detailed topographical studies because each tissue has its own distinctive vascular pattern which delineates it clearly.A direct three‐dimensional inspection has many advantages over the usual methods of dissection, or reconstruction from serial sections. This is particularly true of microcirculations, fine autonomic nerve patterns and the distribution of aberrant tissues (thyroid, parathyroid, etc.). Other distinctive features of tissues, such as positive chromaffin staining or the uptake of injected substance by the reticulo‐endothelial system, can also be demonstrated.The results obtained are demonstrated by photographs of “whole‐mounts” from dogs and adult and fetal cats. The advantages and disadvantages of the method are discussed.