Abstract
The poly- o-phenylenediamine (P oPD) nonconducting film and gold nanoparticles (AuNPs) were combined to fabricate AuNPs/P oPD film, which is used as a novel biocompatible interface for the immobilization of antibody and develop a simple and sensitive label-free immunoassay for the detection of the related antigen (human immunoglobulin G (IgG)). Surface plasmon resonance (SPR) and electrochemical methods were used to provide the real-time information about the polymer film growth, assembling of various sizes of gold nanoparticles, anti-human IgG antibody (anti-hIgG) immobilization and the antigen–antibody interaction. The microstructures of the P oPD and AuNPs/P oPD films were characterized by atomic force microscopy (AFM). These results demonstrated that AuNPs were uniformly dispersed on the porous surface of P oPD film, which formed a nano-structure biocompatible AuNPs/P oPD interface. The use of gold nanoparticles and P oPD film could enhance the immunoassay sensitivity and anti-nonspecific property of the resulting immunoassay electrode. Additionally, the reproducibility and preliminary application of anti-hIgG/AuNPs/P oPD/Au electrode for SPR detection of hIgG was also evaluated.
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