Abstract
A study of alginate-chitosan membrane synthesize was done. The membrane was prepared by mixing alginate hydrosol and chitosan hydrosol at mass ratio of 1:1 and pH of 5.28 approximately. Then it was applied for matrix immobilization of urease and bromothymol blue (BTB) by entrapment technique. The physical, chemical, thermal properties of alginate–chitosan membrane and their impact on immobilized urease activity were investigated. The polymer products were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). It’s showed that alginate-chitosan membrane was formed by electrostatic interaction. The obtained membran has better mechanical properties than original alginat and chitosan membranes. The immobilization urease into alginate-chitosan membrane retained the catalytic activity of the enzyme, as confirmed by color change of BTB indicator after membrane was immersed in substrate solution (urea). Therefore, alginate-chitosan membrane has good characteristics as matrix of enzyme immobilization.
Highlights
The increased interest of scientific and industrial in polyion complex (PIC) membranes implicate in increasing number of its articles
The drying temperature of the alginate-chitosan membrane was optimum at room temperature, even though the aging time needed was longer (72 h) to make alginate-chitosan hydrogel membrane
PIC membrane of alginate–chitosan as urease immobilization matrix was synthesized by mixing both hydrosol of alginate and chitosan at pH of 5.28
Summary
The increased interest of scientific and industrial in polyion complex (PIC) membranes implicate in increasing number of its articles. The membrane was prepared by mixing two polymer solutions consist of chitosan hydrosol and alginate hydrosol, according to the method carried out by Hermanto, Kuswandi, Siswanta, & Mudasir Application membrane for enzyme immobilization The mixtures of alginate-chitosan hydrosol are used as the solid support for the urease and BTB immobilization.
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