Abstract
Polychlorinated biphenyls (PCBs) are a group of synthetic xenobiotics, which were used in lots of industrial applications. Two individual congers (PCB 153 and PCB 77) belong to the most prominent environmental contaminations among PCBs, but they have opposite type of configuration and therefore they can exert a different way of action. Since PCBs increased the force of myometrial contractions, in this work, their effect on myometrial sensitivity on inhibitor of myometrial contractions (relaxine, RLX) was studied. Bovine myometrial cells and strips, and luteal cell at 4-8 month of pregnancy, were obtained accidentally in commercial abattoir. They were treated by PCB 153 and PCB 77 and mRNA expression of RLX receptor in myometrial cells and contractions of myometrial strips as well as the secretion of progesterone (P4) and oxytocin (OT) from luteal cells, were measured under their effect. Both studied congeners decreased (P<0.05) the mRNA expression of RLX receptor in myometrium. Admittedly, both PCB 77 and 153 increased (P<0.05) the basal force of contractions, while RLX showed any effect on myometrial contractions. Studied PCBs increased also (P<0.05) secretion of P4 and OT from luteal cells. It is suggested that PCBs can decreased the sensitivity of myometrium on RLX but their direct effect on RLX function have to be further investigated.
Highlights
Polychlorinated biphenyls (PCBs) are a group of synthetic, chlorinated aryl hydrocarbons, which were extensively used in lots of industrial applications, mainly in transformers, capacitors or as hydraulic fluids, heat exchange liquids as well as plasticizers and others [1]
Almost all length of pregnancy needs uterine quiescence [24,25]. It is reached by progesterone (P4) block of oxytocin (OT) receptors in the myometrium, [26] and relaxin (RLX), which directly inhibit the uterine contractions, as well it is involved in cervical dilation and relaxation of pubic symphysis [27]
The aim of this study was to investigate the effect of PCB 77 and PCB 153 on myometrial reception of RLX signal during pregnancy, and on (a) mRNA expression of receptor of RLX and (b) RLX-inhibition of myometrial strips contractions
Summary
Polychlorinated biphenyls (PCBs) are a group of synthetic, chlorinated aryl hydrocarbons, which were extensively used in lots of industrial applications, mainly in transformers, capacitors or as hydraulic fluids, heat exchange liquids as well as plasticizers and others [1]. Because of different degree of chlorination, there are possible up to 209 congeners of PCBs. Among them, 3,3’,4,4’-tetrachlorobiphenyl (PCB 77) is one of the most abundant PCB congeners in human milk. 3,3’,4,4’-tetrachlorobiphenyl (PCB 77) is one of the most abundant PCB congeners in human milk This congener is a non-ortho chlorine substituted with coplanar configuration and appear to exert its toxic effects via interacting with the cytosolic. Almost all length of pregnancy needs uterine quiescence [24,25]. It is reached by progesterone (P4) block of oxytocin (OT) receptors in the myometrium, [26] and relaxin (RLX), which directly inhibit the uterine contractions, as well it is involved in cervical dilation and relaxation of pubic symphysis [27]. Since it was found previously that PCBs increased amount of stimulators of contractions (OT and PGF2), here it was hypothesized that PCBs can increase the uterine
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