Abstract
BackgroundProtein-protein interaction (PPI) maps are useful tools for investigating the cellular functions of genes. Thus far, large-scale PPI mapping projects have not been implemented for the rice blast fungus Magnaporthe grisea, which is responsible for the most severe rice disease. Inspired by recent advances in PPI prediction, we constructed a PPI map of this important fungus.ResultsUsing a well-recognized interolog approach, we have predicted 11,674 interactions among 3,017 M. grisea proteins. Although the scale of the constructed map covers approximately only one-fourth of the M. grisea's proteome, it is the first PPI map for this crucial organism and will therefore provide new insights into the functional genomics of the rice blast fungus. Focusing on the network topology of proteins encoded by known pathogenicity genes, we have found that pathogenicity proteins tend to interact with higher numbers of proteins. The pathogenicity proteins and their interacting partners in the entire network were then used to construct a subnet called a pathogenicity network. These data may provide further clues for the study of these pathogenicity proteins. Finally, it has been established that secreted proteins in M. grisea interact with fewer proteins. These secreted proteins and their interacting partners were also compiled into a network of secreted proteins, which may be helpful in constructing an interactome between the rice blast fungus and rice.ConclusionWe predicted the PPIs of M. grisea and compiled them into a database server called MPID. It is hoped that MPID will provide new hints as to the functional genomics of this fungus. MPID is available at .
Highlights
Protein-protein interaction (PPI) maps are useful tools for investigating the cellular functions of genes
Using a well-recognized interolog approach, we compiled a draft map of the PPI network in M. grisea, which can be characterized as a "scale-free" network
B that were reported to have an interaction in one model organism; IS( A′i, A) is the InParanoid score between A′i and A, while IS( B′i, B) is the InParanoid score between B′i and B; N is the total number of interologs of protein pair A and B identified in the five model organisms
Summary
Protein-protein interaction (PPI) maps are useful tools for investigating the cellular functions of genes. Large-scale PPI mapping projects have not been implemented for the rice blast fungus Magnaporthe grisea, which is responsible for the most severe rice disease. In the past few years, high-throughput methods have been implemented to identify PPIs [7,8,9,10,11] Using these experimental methods, such as yeast two-hybrid screens, PPI networks for a series of model organisms were determined that allow us to understand the function of proteins at the level of systems biology. None of these high-throughput methods has been applied to the rice blast fungus, despite its importance [12]. A PPI network of M. grisea is urgently required to direct our further investigation of this fungus
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