The prediction and expression of miR-203a-p and miR-29b* against DNMT3B as well as TNFAIP3 in melanoma

Abstract

BackgroundThe development of melanoma could be derived by microRNA (miRNA)-mediated epigenetic regulation of tumor suppressor genes and oncogenes. miRNAs target some genes such as DNA methyltransferase 3-beta (DNMT3B) and consequently, DNMT3B could induce the expression of various genes like TNFAIP3. Therefore, the present study predicted the miRNAs targeting DNMT3B, and also evaluated the expression of TNFAIP3 in the melanoma cell lines. Materials and methodsAnalysis of gene suppression pathways and prediction of the targets of miRNAs including 3′UTR mRNA of DNMT3B gene was performed through various popular algorithms including miRanda, miRDB, miRWalk, RNAhybrid, PICTAR4, PICTAR5, PITA, RNA22, and Targetscan. Total RNA was extracted from the A375 cell line as melanoma cell and melanocyte cell line as a normal cell line. The expression of miRNAs, DNAMT3B, and TNFAIP3 was measured using Real-time PCR. ResultsMore than twenty miRNAs gained the highest scores by targeting the DNMT3B which among them hsa-miR-203a-p and hsa-miR-29b* were selected. A reduction in miRNAs and TNFAIP3 expression was observed according to the Real-time PCR (p-value 0.0323 and fold change 0.656). Also, the expression of DNMT3B increased significantly compared to the control group (p-value 0.0015 and fold change 3.482). ConclusionThe current study exhibited a decrease in the expression of TNFAIP3, miR-203a-pa-p, and miR-29b along with an increase in the expression of DNMT3B. These findings provided a helpful view of a new epigenetic-based approach for the early diagnosis of melanoma.