Abstract
Background Guanylyl cyclase (GC)-G is the last member of the receptor GC family [1,2]. Our recent studies demonstrated that GC-G expressed in Grueneberg Ganglion (GG) neurons can be activated by cool temperatures to generate ultrasound calls by isolated pups to elicit maternal care [3]. Detecting the semiochemical warnings present in the environment is essential for species survival. The mouse GG is the olfactory subsystem that also detects alarm pheromones (APs) and other structurallyrelated chemicals involuntary released by rodent’s predators [4,5]. The predator odor 2,4,5-trimethylthiazoline (TMT), a volatile compound originally isolated from the anal secretions of the red fox, induces robust freezing behaviors in mice. TMT shared a similar chemical structure to APs and can activate GG neurons [5]. However, whether TMT can directly bind and stimulate GC-G activity to trigger innate fear responses remains unknown.
Highlights
Guanylyl cyclase (GC)-G is the last member of the receptor GC family [1,2]
A direct interaction with notable affinity between TMT and GC-G extracellular domain was confirmed by time-resolved surface plasmon resonance
HEK293T cells co-expressing GC-G and the cGMP-activated ion channel CNGA3 respond to TMT via a rapid influx of calcium. In line with these findings, TMT-induced calcium transients in the GG as well as TMT-evoked innate fear behaviors and an increase of serum corticosterone were markedly attenuated in the GC-G-KO mice compared to wild-type littermates
Summary
Guanylyl cyclase (GC)-G is the last member of the receptor GC family [1,2]. Our recent studies demonstrated that GC-G expressed in Grueneberg Ganglion (GG) neurons can be activated by cool temperatures to generate ultrasound calls by isolated pups to elicit maternal care [3]. A direct interaction with notable affinity between TMT and GC-G extracellular domain was confirmed by time-resolved surface plasmon resonance.
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